FastQCFastQC Report
Sun 1 Jan 2017
EPI-120_S32_L005_R1_001_val_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-120_S32_L005_R1_001_val_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24141269
Sequences flagged as poor quality0
Sequence length20-101
%GC23

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTAATATTTGGGTTTTAATGGATATGTTAGTTGTTTTTTATATTTTTTG783320.32447341521276285No Hit
GGTAATATTTGGGTTTTAATGGATATTTTAGTTGTTTTTTATATTTTTTG292040.120971271228534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATGGT7850.061.4938741
CCTAGGT9350.061.30891
CCAAGGT4000.060.340871
CTGTGGT40450.059.7761341
CCTAGGA3250.059.6777761
CTCTGGT12850.059.3681681
CCTGGGT50550.059.343281
CCGTAGC1250.058.6168371
CTTGGGT37300.058.5844881
ACTGGGT28400.058.4286541
CCAGTGG3700.058.2440761
CTTACGC302.1049849E-457.467491
CATGGGT28350.056.7073331
CCGATGT2850.055.9551851
ACTTGGC850.055.777271
CCCGTAG2400.055.6716351
CCGTGGT14300.055.4581381
ACACGCG704.9112714E-1155.4150771
CCAGTAT4400.054.8553351
CTAAGGG6400.054.5492171
Produced by FastQC (version 0.11.4)