FastQCFastQC Report
Sun 1 Jan 2017
EPI-120_S32_L005_R2_001_val_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-120_S32_L005_R2_001_val_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24141269
Sequences flagged as poor quality0
Sequence length20-101
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACATAAACCACAATCAAAAACTAAATATCACTAAACAAACCAAAAAA694880.2878390527026562No Hit
TCTGCATAAACCACAATCAAAAACTAAATATCACTAAACAAACCAAAAAA254370.10536728620189767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCAT55500.074.5228041
CGCAGAT4600.072.043292
CCGCAGA3700.070.9556351
GACCTGC4850.070.104221
GGCCCCT12500.069.206181
ATGCCCT17400.069.0102841
AGTCCGA1400.067.632191
GGCCCGA1850.067.466021
GGATAAC5600.066.094545
CTGCATA68300.065.913082
GCCCAGT3200.065.902961
CCGCTGA2500.065.418771
CACGGAT3200.064.5582662
GGCCCCA11450.063.9002081
CCCGTGA1700.063.2921561
CCCGGAC2250.063.1236422
AGCCCCT15600.062.3508871
GGACCCA3400.062.026311
CCTCAGA6950.061.926141
GCCCGGA1950.061.799111
Produced by FastQC (version 0.11.4)