FastQCFastQC Report
Sun 1 Jan 2017
EPI-127_S33_L005_R1_001_val_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-127_S33_L005_R1_001_val_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22262726
Sequences flagged as poor quality0
Sequence length20-101
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTAATATTTGGGTTTTAATGGATATGTTAGTTGTTTTTTATATTTTTTG478050.21473111603673334No Hit
GGTAATATTTGGGTTTTAATGGATATTTTAGTTGTTTTTTATATTTTTTG248630.11167994431589374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTAGC1300.076.826031
CTAAGGC1300.073.485761
CCTAGGT9450.068.926041
CTATGCG2750.066.319381
CTATGGC2450.065.57821
CTAAGTC200.002960994465.135111
CTTAGGC1300.063.4649771
CCGACGT5350.063.3088871
CCGTGGC2200.063.1613121
CCATGGT8050.062.5728571
CTAGGTC1050.062.0334321
CTACGCG1850.061.0274851
TAAGCCC501.1334123E-860.7927671
CCAAGGT3750.060.2137871
CTGTAAC652.1827873E-1160.1247181
CTGTGGT39250.059.8523861
CCTAGGG6100.059.796161
ACTGGGT27900.059.7655451
CTAGGAC1600.059.707181
CTGTGGC3100.058.8317071
Produced by FastQC (version 0.11.4)