FastQCFastQC Report
Sun 1 Jan 2017
EPI-127_S33_L005_R2_001_val_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-127_S33_L005_R2_001_val_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22262726
Sequences flagged as poor quality0
Sequence length20-101
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACATAAACCACAATCAAAAACTAAATATCACTAAACAAACCAAAAAA470810.2114790434917988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCAT41200.077.040521
CACGGAT3000.075.160452
GCCCAGA3450.074.1546251
GGACCCA3600.073.473821
GGCCCCT12300.072.974411
GGATAAC5250.072.6800165
GGCCCCA12150.070.3064651
GACCTGC4150.070.0054861
CCGCAGA3600.068.655871
CGCAGAT4550.068.616482
GGACTCT700.068.139661
GCAGATA6350.067.602833
GGCCCGT900.067.4513851
ATGCCCT16350.067.097781
CCTCAGA6800.066.9554141
GACCAGA3250.066.710161
GGCCGCA2800.066.5910341
TGCCAGA1050.066.074831
CCACGGA3850.065.323981
AGCCCCT14150.065.272231
Produced by FastQC (version 0.11.4)