FastQCFastQC Report
Sun 1 Jan 2017
EPI-128_S34_L005_R1_001_val_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-128_S34_L005_R1_001_val_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24559031
Sequences flagged as poor quality0
Sequence length20-101
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTAATATTTGGGTTTTAATGGATATGTTAGTTGTTTTTTATATTTTTTG475000.1934115397305374No Hit
GGTAATATTTGGGTTTTAATGGATATTTTAGTTGTTTTTTATATTTTTTG250960.10218644212794878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGGC2850.064.822371
CCTAGGT9650.062.7759251
CCGACGT6200.060.9806861
CTGACGT2050.060.7778631
CCTGGGT56550.060.24791
CCGTGGC1900.058.792381
CCCGTGT5150.058.3972131
ACTGGGT33250.058.0170971
CTGTGGT42750.057.8878821
CTAGCGA751.8189894E-1257.284891
CTTAGGC1750.056.4665341
CATGGGT28450.056.177451
CTTGGGT40350.056.0071561
ACTGGGG17400.055.8033871
CCGTAGC1700.055.6000371
CCGTAGT9050.055.544191
CCGACGA4800.055.494741
CTCCGCG705.0931703E-1155.2390021
CTGACGA1400.055.2390021
CCTGGTA44050.055.2041661
Produced by FastQC (version 0.11.4)