FastQCFastQC Report
Sun 1 Jan 2017
EPI-128_S34_L005_R2_001_val_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-128_S34_L005_R2_001_val_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24559031
Sequences flagged as poor quality0
Sequence length20-101
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACATAAACCACAATCAAAAACTAAATATCACTAAACAAACCAAAAAA483480.19686444469246364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCAT44950.071.834811
CGCAGAT5750.070.8480762
CCGCAGA5250.069.427131
GGCCCCT14200.066.436071
GCAGATA9100.065.02973
ATGCCCT19000.064.547891
CCACGGA3400.064.32221
GTCCGCA5950.060.538541
AGATAAC37600.060.1015055
TGCCCCT21700.060.073571
CTGCATA59300.059.6583862
CAGATAA44500.059.6483574
ACGGCCA2600.059.3743361
GCCCCGT5750.058.169641
CACGGAT3100.058.0978242
AGCCCGA3100.058.097471
GGACCTA3100.058.097471
GTCCCGT3550.057.9805761
GACCTGC3500.057.583681
ACGGATA3950.057.5377463
Produced by FastQC (version 0.11.4)