FastQCFastQC Report
Sun 1 Jan 2017
EPI-135_S35_L005_R1_001_val_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-135_S35_L005_R1_001_val_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28732790
Sequences flagged as poor quality0
Sequence length20-101
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTAATATTTGGGTTTTAATGGATATGTTAGTTGTTTTTTATATTTTTTG460830.16038470333023697No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGGT11300.065.2426451
CCATGGT10300.062.0332951
CCTAGCG402.5289773E-761.436831
CCGTAGC1400.061.4368251
ACTAGGC950.060.3589821
CTAGACG2150.059.0555531
CCGTGGC1900.058.2033041
CCTACGT1200.058.0236661
CTAAGGG8500.057.822891
CCTGGGT65450.057.697731
CTAAGGC2650.057.1864781
CTGTGGC3450.056.984881
CCGTAGT9850.056.9668051
CTAGGAC1950.056.710921
TCCGTGC654.0017767E-1156.710911
CTATGCG3700.056.4554631
ACTGGGT38100.056.115521
CCGTAAC950.056.0476261
CAGTGGT36050.056.0117531
CTTGGGT44900.055.553121
Produced by FastQC (version 0.11.4)