FastQCFastQC Report
Sun 1 Jan 2017
EPI-135_S35_L005_R2_001_val_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-135_S35_L005_R2_001_val_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28732790
Sequences flagged as poor quality0
Sequence length20-101
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACATAAACCACAATCAAAAACTAAATATCACTAAACAAACCAAAAAA513240.1786251874600413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCAT43900.070.392761
GGATAAC6800.069.219025
GCCCAGT4150.069.039161
GGCCCCT15700.067.782771
CGCAGAT6750.067.307822
GACCTGC4450.067.144181
CCGCAGA6100.067.098951
ATGCCCT22350.065.561831
GCAGATA9250.065.489153
AGCCCCT18800.065.314411
GGCCGCT1600.063.953691
GCCATGA4750.063.7651981
CGACTGT1100.063.2560161
CGGATAA6300.063.019324
GGCCCCA14750.062.4361461
GCCTAGC1800.061.3955461
GGTCCCA4150.061.148971
CAGCTTG600.003835691461.0157794-95
GGTCCAA3050.060.3890531
GCCCCGT7400.060.2893181
Produced by FastQC (version 0.11.4)