FastQCFastQC Report
Sun 1 Jan 2017
EPI-136_S36_L005_R1_001_val_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-136_S36_L005_R1_001_val_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27793041
Sequences flagged as poor quality0
Sequence length20-101
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTAATATTTGGGTTTTAATGGATATGTTAGTTGTTTTTTATATTTTTTG685990.24682077790623919No Hit
GGTAATATTTGGGTTTTAATGGATATTTTAGTTGTTTTTTATATTTTTTG466120.16771104680484586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGGC3100.064.031851
CTAAGGC1950.063.6213951
CCTAGGT9400.059.831191
CTCTGGT16700.058.1926231
CCTGGGT60250.057.655241
ACTGGGT33800.057.2592621
CTGTGGT42150.057.1007651
CCATGGT10950.057.0268481
CCTAGAA1600.056.8616261
CATAGAC950.056.5895581
CCATGTA3150.056.4513661
CCTAGTG3850.055.854631
CAATGGG8250.055.63981
CCTGTGT7550.055.321121
CTCTGGG13100.055.2437741
ACTAGGC1050.055.1385461
CTTAGGC1500.055.1385461
CTTAGCG1800.055.1385461
CCTAGGG5000.054.587161
CTCAGGT3350.054.315581
Produced by FastQC (version 0.11.4)