FastQCFastQC Report
Sun 1 Jan 2017
EPI-136_S36_L005_R2_001_val_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-136_S36_L005_R2_001_val_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27793041
Sequences flagged as poor quality0
Sequence length20-101
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACATAAACCACAATCAAAAACTAAATATCACTAAACAAACCAAAAAA882140.3173959985163192No Hit
TCTGCATAAACCACAATCAAAAACTAAATATCACTAAACAAACCAAAAAA305140.10979007298985383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCAT62700.074.137051
CACGGAT2750.070.618132
GGCCCCT14100.070.037251
ATGCCCT20500.069.133821
GCCTAGC1350.067.334751
CGCAGAT5900.067.231112
GCAGATA8250.067.112633
GACTAGA1750.066.110471
GGACCCT2950.065.830351
GGACCCC1200.065.421831
CTGCATA78100.064.544592
GGATAAC5750.063.954245
GCCCAGA4850.063.895441
GCCATGA4500.063.3558731
GGACCCA4150.062.72531
AGCCCCT18200.062.20561
CCGCAGA5700.061.6161231
GCCCAGT5050.061.3649251
TGCATAA81950.060.6554833
GGCTCCA3300.060.1004331
Produced by FastQC (version 0.11.4)