FastQCFastQC Report
Sun 1 Jan 2017
EPI-143_S37_L005_R1_001_val_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-143_S37_L005_R1_001_val_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21162099
Sequences flagged as poor quality0
Sequence length20-101
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTAATATTTGGGTTTTAATGGATATGTTAGTTGTTTTTTATATTTTTTG339720.16053227990285843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGGC1850.069.420961
CCTAGGT8600.064.712171
CTGTACG2300.063.283741
CCAAGGT4300.060.7298851
CCATGGT6400.060.2009851
CCTGGGT49900.059.2814141
CTGACGT1900.058.5815471
CTGTGGT36100.058.462961
CTAAGGG5850.057.8112371
CCGTAGT8250.057.598361
CTTAGGC1650.057.0794531
ACTGGGT27250.057.0270841
ACTGGTA14800.056.9830361
CCGAGGT5250.056.264031
CCGTGGT14750.056.015261
CTAAGGC1300.055.981771
ACTGGTG13950.055.545061
CTAAGGT9750.055.3231621
CCTGGGA16450.055.171021
CTATGGT19300.054.7874031
Produced by FastQC (version 0.11.4)