FastQCFastQC Report
Sun 1 Jan 2017
EPI-143_S37_L005_R2_001_val_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-143_S37_L005_R2_001_val_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21162099
Sequences flagged as poor quality0
Sequence length20-101
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACATAAACCACAATCAAAAACTAAATATCACTAAACAAACCAAAAAA333070.1573898695020754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCAT34900.071.983691
GGTCCCT2100.069.1738361
GGCCCCT12300.068.776851
GACCTGC3550.067.39721
GCAGATA7250.067.181873
GGACCAT2600.065.730791
GGCCCCA10550.064.796231
CGCAGAT4900.064.523792
ATGCCCT16250.063.6274031
GCCCAGT3450.063.158711
GGACCCT2100.063.070261
GGATAAC4250.062.3272255
GACCAGA2850.061.4640541
CATGGAT1950.061.3490222
GACCAGT1750.061.0357361
AGATAAC32300.060.581215
GGTGTGC20800.060.386456
TGTGCTC25650.060.2966428
CTGCATA46800.059.8883362
CCACAGA13850.059.8458671
Produced by FastQC (version 0.11.4)