FastQCFastQC Report
Sun 1 Jan 2017
EPI-145_S38_L005_R1_001_val_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-145_S38_L005_R1_001_val_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25315253
Sequences flagged as poor quality0
Sequence length20-101
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTAATATTTGGGTTTTAATGGATATGTTAGTTGTTTTTTATATTTTTTG451950.17852873127517233No Hit
GGTAATATTTGGGTTTTAATGGATATTTTAGTTGTTTTTTATATTTTTTG253190.10001480135316049No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCCC159.87138E-485.9105151
CCTAGGT9700.069.525531
TCCTAGG1300.069.3892751
CCTAGGC1000.068.728421
CTATGGC2700.068.410231
CCATGGT8750.066.764751
CCCGGTC650.066.085021
CTCTGGT13350.064.995981
GCTAGTC200.00309096564.43291
CTCAGGT2800.064.432891
ACCTAGG750.063.0010531
ACTGGGT32100.062.7601781
CCAAGGT4450.062.7436451
CCTAGGG6050.062.480381
CAATGGC1650.062.480381
CCAGTAG3200.061.748191
CCGTGGC1700.060.6427231
CTCGCGG1700.060.6427231
CCGATGT3550.060.500371
CTGTGGT40300.060.116051
Produced by FastQC (version 0.11.4)