FastQCFastQC Report
Sun 1 Jan 2017
EPI-145_S38_L005_R2_001_val_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-145_S38_L005_R2_001_val_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25315253
Sequences flagged as poor quality0
Sequence length20-101
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACATAAACCACAATCAAAAACTAAATATCACTAAACAAACCAAAAAA498720.197003758959075No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCAT42350.074.7390751
GACCTGC3150.073.5237961
GGTCCGT1000.072.91111
GGACCGC600.071.481471
CGCAGAT6150.070.4356842
GGACCCA4250.069.631361
GGCCCCA12250.069.322441
ATGCCCT17600.068.963371
GCCCAGT3750.068.6222151
CAAGGTA501.6189006E-1068.6222151
CCGCAGA5200.068.457261
GCAGATA8900.068.428783
GGCCCCT13050.067.3733441
GGATAAC5950.066.3132255
GACCAGA3900.064.883191
CTCTGCG200.003109916864.333582
TAGGTCC200.003109965564.333331
ACGTGCC800.064.333331
AGCCCCT17300.063.7135471
CACGGAT2900.063.5941162
Produced by FastQC (version 0.11.4)