Bismark report for: /Volumes/web/nightingales/C_virginica/zr2096_1_s1_R2.fastq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /Users/yaamini/Documents/project-virginica-oa/analyses/2018-04-27-Bismark/2018-04-27-Bismark-Inputs/ with the specified options: -q --score-min L,0,-1.2 --ignore-quals Final Alignment report ====================== Sequences analysed in total: 10000 Number of alignments with a unique best hit from the different alignments: 3211 Mapping efficiency: 32.1% Sequences with no alignments under any condition: 5683 Sequences did not map uniquely: 1106 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 756 ((converted) top strand) CT/GA: 690 ((converted) bottom strand) GA/CT: 906 (complementary to (converted) top strand) GA/GA: 859 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 52598 Total methylated C's in CpG context: 4485 Total methylated C's in CHG context: 417 Total methylated C's in CHH context: 2492 Total methylated C's in Unknown context: 324 Total unmethylated C's in CpG context: 2134 Total unmethylated C's in CHG context: 10794 Total unmethylated C's in CHH context: 32276 Total unmethylated C's in Unknown context: 1129 C methylated in CpG context: 67.8% C methylated in CHG context: 3.7% C methylated in CHH context: 7.2% C methylated in Unknown context (CN or CHN): 22.3% Bismark completed in 0d 0h 1m 12s