Bismark report for: /Volumes/web/nightingales/C_virginica/zr2096_2_s1_R2.fastq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /Users/yaamini/Documents/project-virginica-oa/analyses/2018-04-27-Bismark/2018-04-27-Bismark-Inputs/ with the specified options: -q --score-min L,0,-1.2 --ignore-quals Final Alignment report ====================== Sequences analysed in total: 10000 Number of alignments with a unique best hit from the different alignments: 5481 Mapping efficiency: 54.8% Sequences with no alignments under any condition: 2531 Sequences did not map uniquely: 1988 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 1295 ((converted) top strand) CT/GA: 1317 ((converted) bottom strand) GA/CT: 1452 (complementary to (converted) top strand) GA/GA: 1417 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 87388 Total methylated C's in CpG context: 8135 Total methylated C's in CHG context: 666 Total methylated C's in CHH context: 3294 Total methylated C's in Unknown context: 448 Total unmethylated C's in CpG context: 3627 Total unmethylated C's in CHG context: 19502 Total unmethylated C's in CHH context: 52164 Total unmethylated C's in Unknown context: 1554 C methylated in CpG context: 69.2% C methylated in CHG context: 3.3% C methylated in CHH context: 5.9% C methylated in Unknown context (CN or CHN): 22.4% Bismark completed in 0d 0h 1m 31s