Bismark report for: /Volumes/web/nightingales/C_virginica/zr2096_4_s1_R1.fastq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /Users/yaamini/Documents/project-virginica-oa/analyses/2018-04-27-Bismark/2018-04-27-Bismark-Inputs/ with the specified options: -q --score-min L,0,-1.2 --ignore-quals Final Alignment report ====================== Sequences analysed in total: 10000 Number of alignments with a unique best hit from the different alignments: 6169 Mapping efficiency: 61.7% Sequences with no alignments under any condition: 1547 Sequences did not map uniquely: 2284 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 1561 ((converted) top strand) CT/GA: 1508 ((converted) bottom strand) GA/CT: 1514 (complementary to (converted) top strand) GA/GA: 1586 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 99485 Total methylated C's in CpG context: 8450 Total methylated C's in CHG context: 690 Total methylated C's in CHH context: 2940 Total methylated C's in Unknown context: 390 Total unmethylated C's in CpG context: 4523 Total unmethylated C's in CHG context: 21587 Total unmethylated C's in CHH context: 61295 Total unmethylated C's in Unknown context: 1734 C methylated in CpG context: 65.1% C methylated in CHG context: 3.1% C methylated in CHH context: 4.6% C methylated in Unknown context (CN or CHN): 18.4% Bismark completed in 0d 0h 2m 2s