Bismark report for: /Volumes/web/nightingales/C_virginica/zr2096_5_s1_R2.fastq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /Users/yaamini/Documents/project-virginica-oa/analyses/2018-04-27-Bismark/2018-04-27-Bismark-Inputs/ with the specified options: -q --score-min L,0,-1.2 --ignore-quals Final Alignment report ====================== Sequences analysed in total: 10000 Number of alignments with a unique best hit from the different alignments: 6202 Mapping efficiency: 62.0% Sequences with no alignments under any condition: 1537 Sequences did not map uniquely: 2261 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 1394 ((converted) top strand) CT/GA: 1366 ((converted) bottom strand) GA/CT: 1706 (complementary to (converted) top strand) GA/GA: 1736 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 103940 Total methylated C's in CpG context: 8868 Total methylated C's in CHG context: 757 Total methylated C's in CHH context: 2856 Total methylated C's in Unknown context: 433 Total unmethylated C's in CpG context: 4481 Total unmethylated C's in CHG context: 22166 Total unmethylated C's in CHH context: 64812 Total unmethylated C's in Unknown context: 1860 C methylated in CpG context: 66.4% C methylated in CHG context: 3.3% C methylated in CHH context: 4.2% C methylated in Unknown context (CN or CHN): 18.9% Bismark completed in 0d 0h 2m 29s