Bismark report for: /Volumes/web/nightingales/C_virginica/zr2096_2_s1_R1.fastq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /Users/yaamini/Documents/project-virginica-oa/analyses/2018-04-27-Bismark/2018-04-27-Bismark-Inputs/ with the specified options: -q --score-min L,0,-1.2 --ignore-quals Final Alignment report ====================== Sequences analysed in total: 30798582 Number of alignments with a unique best hit from the different alignments: 16947236 Mapping efficiency: 55.0% Sequences with no alignments under any condition: 7672436 Sequences did not map uniquely: 6178910 Sequences which were discarded because genomic sequence could not be extracted: 5 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 4124744 ((converted) top strand) CT/GA: 4152670 ((converted) bottom strand) GA/CT: 4327789 (complementary to (converted) top strand) GA/GA: 4342028 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 271024981 Total methylated C's in CpG context: 25747003 Total methylated C's in CHG context: 2080992 Total methylated C's in CHH context: 9553145 Total methylated C's in Unknown context: 1213922 Total unmethylated C's in CpG context: 11120086 Total unmethylated C's in CHG context: 60523320 Total unmethylated C's in CHH context: 162000435 Total unmethylated C's in Unknown context: 4791818 C methylated in CpG context: 69.8% C methylated in CHG context: 3.3% C methylated in CHH context: 5.6% C methylated in Unknown context (CN or CHN): 20.2% Bismark completed in 0d 13h 59m 27s