Bismark report for: /Volumes/web/nightingales/C_virginica/zr2096_4_s1_R2.fastq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /Users/yaamini/Documents/project-virginica-oa/analyses/2018-04-27-Bismark/2018-04-27-Bismark-Inputs/ with the specified options: -q --score-min L,0,-1.2 --ignore-quals Final Alignment report ====================== Sequences analysed in total: 24341968 Number of alignments with a unique best hit from the different alignments: 15266792 Mapping efficiency: 62.7% Sequences with no alignments under any condition: 3502517 Sequences did not map uniquely: 5572659 Sequences which were discarded because genomic sequence could not be extracted: 8 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 3473421 ((converted) top strand) CT/GA: 3483033 ((converted) bottom strand) GA/CT: 4147234 (complementary to (converted) top strand) GA/GA: 4163096 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 245364335 Total methylated C's in CpG context: 20716861 Total methylated C's in CHG context: 1725952 Total methylated C's in CHH context: 6966764 Total methylated C's in Unknown context: 893665 Total unmethylated C's in CpG context: 11250057 Total unmethylated C's in CHG context: 53936811 Total unmethylated C's in CHH context: 150767890 Total unmethylated C's in Unknown context: 4262000 C methylated in CpG context: 64.8% C methylated in CHG context: 3.1% C methylated in CHH context: 4.4% C methylated in Unknown context (CN or CHN): 17.3% Bismark completed in 1d 5h 19m 47s