Bismark report for: /Volumes/web/nightingales/C_virginica/zr2096_5_s1_R1.fastq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /Users/yaamini/Documents/project-virginica-oa/analyses/2018-04-27-Bismark/2018-04-27-Bismark-Inputs/ with the specified options: -q --score-min L,0,-1.2 --ignore-quals Final Alignment report ====================== Sequences analysed in total: 31778715 Number of alignments with a unique best hit from the different alignments: 19730827 Mapping efficiency: 62.1% Sequences with no alignments under any condition: 5053315 Sequences did not map uniquely: 6994573 Sequences which were discarded because genomic sequence could not be extracted: 25 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 4704514 ((converted) top strand) CT/GA: 4707771 ((converted) bottom strand) GA/CT: 5166758 (complementary to (converted) top strand) GA/GA: 5151759 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 328798878 Total methylated C's in CpG context: 28007894 Total methylated C's in CHG context: 2440790 Total methylated C's in CHH context: 9555686 Total methylated C's in Unknown context: 1397495 Total unmethylated C's in CpG context: 14164169 Total unmethylated C's in CHG context: 70203884 Total unmethylated C's in CHH context: 204426455 Total unmethylated C's in Unknown context: 5853556 C methylated in CpG context: 66.4% C methylated in CHG context: 3.4% C methylated in CHH context: 4.5% C methylated in Unknown context (CN or CHN): 19.3% Bismark completed in 1d 9h 3m 34s