Bismark report for: /Volumes/web/nightingales/C_virginica/zr2096_6_s1_R1.fastq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /Users/yaamini/Documents/project-virginica-oa/analyses/2018-04-27-Bismark/2018-04-27-Bismark-Inputs/ with the specified options: -q --score-min L,0,-1.2 --ignore-quals Final Alignment report ====================== Sequences analysed in total: 24237290 Number of alignments with a unique best hit from the different alignments: 15427827 Mapping efficiency: 63.7% Sequences with no alignments under any condition: 3111552 Sequences did not map uniquely: 5697911 Sequences which were discarded because genomic sequence could not be extracted: 2 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 3844741 ((converted) top strand) CT/GA: 3861066 ((converted) bottom strand) GA/CT: 3858792 (complementary to (converted) top strand) GA/GA: 3863226 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 250829021 Total methylated C's in CpG context: 23883245 Total methylated C's in CHG context: 1839758 Total methylated C's in CHH context: 7204945 Total methylated C's in Unknown context: 1018995 Total unmethylated C's in CpG context: 9489687 Total unmethylated C's in CHG context: 54793502 Total unmethylated C's in CHH context: 153617884 Total unmethylated C's in Unknown context: 4447030 C methylated in CpG context: 71.6% C methylated in CHG context: 3.2% C methylated in CHH context: 4.5% C methylated in Unknown context (CN or CHN): 18.6% Bismark completed in 1d 15h 39m 41s