Bismark report for: /Volumes/web/nightingales/C_virginica/zr2096_7_s1_R1.fastq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /Users/yaamini/Documents/project-virginica-oa/analyses/2018-04-27-Bismark/2018-04-27-Bismark-Inputs/ with the specified options: -q --score-min L,0,-1.2 --ignore-quals Final Alignment report ====================== Sequences analysed in total: 29534746 Number of alignments with a unique best hit from the different alignments: 18779341 Mapping efficiency: 63.6% Sequences with no alignments under any condition: 4078045 Sequences did not map uniquely: 6677360 Sequences which were discarded because genomic sequence could not be extracted: 19 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 4367687 ((converted) top strand) CT/GA: 4383489 ((converted) bottom strand) GA/CT: 5014850 (complementary to (converted) top strand) GA/GA: 5013296 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 316632174 Total methylated C's in CpG context: 28592233 Total methylated C's in CHG context: 2427054 Total methylated C's in CHH context: 8929915 Total methylated C's in Unknown context: 1253887 Total unmethylated C's in CpG context: 12027597 Total unmethylated C's in CHG context: 68723073 Total unmethylated C's in CHH context: 195932302 Total unmethylated C's in Unknown context: 5574713 C methylated in CpG context: 70.4% C methylated in CHG context: 3.4% C methylated in CHH context: 4.4% C methylated in Unknown context (CN or CHN): 18.4% Bismark completed in 1d 22h 1m 13s