Bismark report for: /Users/hollie/Documents/Oly_Oyster_DNA_Methylation/Data/MBD/Clean_Joined_Data/zr1394_1_cleaned_all.fastq (version: v0.16.3) Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Bismark was run with Bowtie 2 against the bisulfite genome of /Users/hollie/Documents/Oly_Oyster_DNA_Methylation/Data/Genome/ with the specified options: -q --score-min L,0,-0.2 --ignore-quals Final Alignment report ====================== Sequences analysed in total: 62868247 Number of alignments with a unique best hit from the different alignments: 1713170 Mapping efficiency: 2.7% Sequences with no alignments under any condition: 59571125 Sequences did not map uniquely: 1583952 Sequences which were discarded because genomic sequence could not be extracted: 229 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 885380 ((converted) top strand) CT/GA: 827561 ((converted) bottom strand) GA/CT: 0 (complementary to (converted) top strand) GA/GA: 0 (complementary to (converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 16177545 Total methylated C's in CpG context: 2464619 Total methylated C's in CHG context: 87107 Total methylated C's in CHH context: 653157 Total methylated C's in Unknown context: 355 Total unmethylated C's in CpG context: 630259 Total unmethylated C's in CHG context: 3197432 Total unmethylated C's in CHH context: 9144971 Total unmethylated C's in Unknown context: 2679 C methylated in CpG context: 79.6% C methylated in CHG context: 2.7% C methylated in CHH context: 6.7% C methylated in Unknown context (CN or CHN): 11.7%