FastQCFastQC Report
Mon 29 Jan 2018
NR005_S4_L002_R1_001_val_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNR005_S4_L002_R1_001_val_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36456
Sequences flagged as poor quality0
Sequence length20-151
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC2730.748847926267281TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCGCGTATGC790.21669958305903006TruSeq Adapter, Index 5 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)