FastQCFastQC Report
Tue 27 Mar 2018
160103_I137_FCH3V5YBBXX_L3_WHPANwalDDAADWAAPEI-101_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename160103_I137_FCH3V5YBBXX_L3_WHPANwalDDAADWAAPEI-101_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences62782565
Sequences flagged as poor quality0
Sequence length49
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTC68550.012.42013129
CCCGTCA69750.012.17562630
TCACCCG72900.011.85596727
CGGAAGA76150.011.6887721
CGTCACG72150.011.59182332
GAACTCC79550.011.05405418
GCACACG84250.010.8201788
GATCTCG73200.010.691256538
CGATCTC73250.010.59590437
CGTATGC82550.010.41792943
CACCCGT88850.010.16319728
GAAGAGC94450.09.6744313
CCGTCAC93700.09.47652131
GTCACGA91750.09.2561333
TCGGAAG62100.08.93236643
ACGATCT88650.08.73096536
GTCACCC102950.08.62506126
ACGTCTG110800.08.20803312
CACGTCT113850.08.15810311
TCTCGTA99300.08.14098640
Produced by FastQC (version 0.11.5)