FastQCFastQC Report
Tue 27 Mar 2018
160103_I137_FCH3V5YBBXX_L5_WHPANwalDDABDLAAPEI-100_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename160103_I137_FCH3V5YBBXX_L5_WHPANwalDDABDLAAPEI-100_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences75775638
Sequences flagged as poor quality0
Sequence length49
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAGC68900.010.23512332
CGGAAGA85650.08.3339171
TCGTAAG87350.08.245563531
GCACACG86650.08.2377388
CGTATGC88850.08.17895343
GAACTCC85750.08.17376118
TCACTCG97900.07.708376427
CTCGTAA96850.07.680949730
GAAGAGC100600.07.2877733
CACTCGT108900.06.91000928
TCTCGTA106300.06.350893540
ACTCGTA122450.06.30338929
CATCTCG110050.06.251703738
TCGTATG126850.06.10169542
CTCGTAT121850.06.052113541
CGTCTGA131100.05.854691513
ATCTCGT123850.05.746063739
ACGTCTG131500.05.706083312
GTCACTC138150.05.695982526
AGAGCAC130100.05.6848585
Produced by FastQC (version 0.11.5)