FastQCFastQC Report
Tue 27 Mar 2018
160103_I137_FCH3V5YBBXX_L5_WHPANwalDDABDLAAPEI-100_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename160103_I137_FCH3V5YBBXX_L5_WHPANwalDDABDLAAPEI-100_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences75775638
Sequences flagged as poor quality0
Sequence length49
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTACG63600.012.5754711
GCGTACG39000.010.143591
ACGAGGG53050.09.8482565
CGAGGGA43150.08.6697576
CTTACGA86750.08.5999992
GCGCTTC34050.07.82966230
CGTACGA47350.07.58289342
CGAGGGG41300.07.28813556
TTACGAG88700.07.2474633
GTGCGCT36350.07.15680928
TACGAGG58250.06.75450664
GCGCCTC23450.06.692963630
TCGGAAG84400.06.597748843
CGCGTGC28850.06.18544225
GCCTACG41100.05.96350341
CGGGGGG52450.05.820781713
CGCGGGC17700.05.709039725
GATCGGA110250.05.55782343
CGTGTGC73850.05.531482725
CGGGGGC34300.05.453352525
Produced by FastQC (version 0.11.5)