FastQCFastQC Report
Wed 28 Mar 2018
Geoduck-NMP-gDNA-1_S1_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-NMP-gDNA-1_S1_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences368469262
Sequences flagged as poor quality0
Sequence length35-151
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3931710710.670389922511365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1404350.09.8992321
CGGAAGA1576100.09.1454794
TCGGAAG1599050.09.0104813
GAAGAGC1665850.08.6038046
ATCGGAA2064350.07.3009332
CGGGGGG223650.07.298492145
AGAGCAC2030200.07.24200778
CGGCGCG108250.07.2019377145
CGCGGGG136350.06.4324236145
GGAAGAG2343250.06.4297575
CCGCGGG154100.06.165797145
GGCGCGG160250.06.081199145
GAGCACA2595450.05.8315619
GGTCGCG219900.05.7611145
AAGAGCA2718000.05.66555177
GCGGGGG388550.05.5804644145
GCCGGCG176400.05.524445145
CCCGACG247200.05.4205265145
CCCCCCG310350.05.416573145
CCCCGCG153753.45608E-115.3875465145
Produced by FastQC (version 0.11.5)