FastQCFastQC Report
Wed 28 Mar 2018
Geoduck-NMP-gDNA-1_S1_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-NMP-gDNA-1_S1_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences368469262
Sequences flagged as poor quality0
Sequence length35-151
%GC36

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN342641349.299048125213766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGG893050.010.969196145
CGCGCGG442150.010.62828145
CCGCGGG429700.010.609766145
CGGGGGG1057300.010.525561145
GCGCGGG556900.010.327481145
GGCGCGG609200.010.170036145
GCGCCGG571750.010.018356145
TCTCGGG684450.09.905875145
GCGGGGG1243200.09.553429145
CGCGGGG563300.09.338548145
CGGCGGG826750.09.218918145
TCGCCGG642650.08.949467145
CCGCCGG536200.08.76407145
AGGGGGG1541300.08.631011145
GCGCGCG607250.08.508662145
CCGCGCG485900.08.420302145
CGCGCCG524300.08.294107145
CCGGCGG521250.08.252933145
GCGTGGG646900.08.240133145
GCGAGGG584500.08.239849145
Produced by FastQC (version 0.11.5)