FastQCFastQC Report
Wed 28 Mar 2018
Geoduck-NMP-gDNA-2_S5_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-NMP-gDNA-2_S5_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377072437
Sequences flagged as poor quality0
Sequence length35-151
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3992536910.588249122011534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1427700.011.0914761
CGGAAGA1610500.09.9867544
TCGGAAG1619350.09.9802573
GAAGAGC1699300.09.5720886
ATCGGAA2088250.08.037972
AGAGCAC2055400.07.9533928
GCGCGGG152950.07.937985145
CGCGGGG156650.07.7504935145
GGAAGAG2414300.06.96098045
CCGCCGG202350.06.720082145
CGCGCGG127750.06.462607145
CGGGGGG231150.06.4080467145
GAGCACA2654950.06.38678179
GGCGCGG160850.06.340419145
AAGAGCA2772450.06.2950547
GCCCGGG265150.06.044245145
CCCCCGG292900.05.9690175145
CCGCGGG160500.05.9003706145
CCGGGGG273250.05.7762094145
GCCCGCG147151.8189894E-125.775605145
Produced by FastQC (version 0.11.5)