FastQCFastQC Report
Wed 28 Mar 2018
Geoduck-NMP-gDNA-2_S5_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-NMP-gDNA-2_S5_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377072437
Sequences flagged as poor quality0
Sequence length35-151
%GC35

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN355909129.438746645913024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGG395800.011.30647145
CGGTGGG877950.010.995041145
CGGGGGG959400.010.720974145
GCGGGGG1137150.010.672875145
GCGCGGG496750.010.470913145
CCGCCGG519800.010.457337145
CGGCGGG785400.010.083127145
CCGGCGG496800.010.045404145
GCGCCGG550500.010.044403145
TCGCCGG651650.09.995392145
GGCGCGG543600.09.956391145
TCTCGGG714650.09.933874145
CGCGGGG523450.09.847295145
CCGCGGG407550.09.715725145
CCCGCGG427900.09.4179325145
ACGGGGG653050.09.14878145
ACGCGGG425950.09.076007145
CCGGGGG657450.08.802452145
GCGAGGG553550.08.549957145
CCCCCGG699350.08.476086145
Produced by FastQC (version 0.11.5)