FastQCFastQC Report
Wed 28 Mar 2018
Geoduck-NMP-gDNA-2to4kb-3_S10_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-NMP-gDNA-2to4kb-3_S10_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences355466683
Sequences flagged as poor quality0
Sequence length35-151
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3655882810.284741087816661No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGG150500.07.851394145
CGGGGGG242000.07.8328066145
CCGCGGG166600.07.535936145
CGCGCGG122950.06.807575145
CGCGGGG144200.06.487246145
CGCGCCG104703.583409E-106.3483243145
GCGGGGG403450.05.979686145
CTGCGGG290700.05.8431373145
CGCAGGG285700.05.7730675145
CCCCGGG387550.05.7168503145
CGGCGCG104659.265568E-85.6456513145
CCGGCGG209750.05.6335387145
GCCCGCG153855.456968E-125.6003165145
CCGCCGG206850.05.5935097145
CGGTGGG370200.05.5857944145
CGAGGGG339850.05.577576145
CGACGGG284350.05.540753145
ACGGGGG329100.05.5353603145
CCGGGGG272250.05.515743145
GCCGGCG197600.05.481606145
Produced by FastQC (version 0.11.5)