FastQCFastQC Report
Wed 28 Mar 2018
Geoduck-NMP-gDNA-2to4kb-3_S10_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-NMP-gDNA-2to4kb-3_S10_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences355466683
Sequences flagged as poor quality0
Sequence length35-151
%GC36

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN328254129.234455314620863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGG347100.013.302931145
CGGGGGG1037950.012.93732145
GCGCGGG465600.012.3459145
GCGGGGG1174200.011.135169145
CCGCGGG379000.011.126529145
CGGTGGG989050.011.099759145
TCGCCGG748850.011.042264145
GCGCCGG555400.010.986004145
TCTCGGG736250.010.719274145
TCGCGGG413350.010.54386145
GGCGCGG537450.010.432413145
TGCGCGG365050.010.067419145
AGGGGGG1602250.09.939448145
CCGGGGG658050.09.88089145
CGCGCCG429800.09.811435145
CCGCCGG487150.09.720289145
CGCGGGG510250.09.557256145
CACGCGG430150.09.420075145
CGGCGGG840300.09.33589145
CGGCGCG420200.09.3067465145
Produced by FastQC (version 0.11.5)