FastQCFastQC Report
Wed 28 Mar 2018
Geoduck-NMP-gDNA-2to4kb-4_S14_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-NMP-gDNA-2to4kb-4_S14_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360317079
Sequences flagged as poor quality0
Sequence length35-151
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3695723410.256864343641062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGG239650.08.630312145
CGCGGGG149100.07.5963473145
CGCGCGG126450.07.5939965145
CCCGCGG171400.07.46994145
GCGCCCG179150.06.8719163145
CCGCCGG211650.06.6310334145
GGCCGCG188300.06.276471145
CCCCCCG371800.06.2250533145
AGGGGGG687850.06.121065145
CCCCCGG323050.06.0974026145
ACGGGGG341450.06.057268145
GCGGCCG212900.06.0138464145
GCCCGCG156350.05.9842587145
GCCCGGG278400.05.925571145
GCGGGGG404300.05.907349145
CCCGCCG398150.05.8749146145
CCGGGGG286050.05.853176145
GCGCGGG152250.05.821968145
CCGGCGG212000.05.807093145
CCCCGCG170300.05.783223145
Produced by FastQC (version 0.11.5)