FastQCFastQC Report
Wed 28 Mar 2018
Geoduck-NMP-gDNA-2to4kb-4_S14_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-NMP-gDNA-2to4kb-4_S14_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360317079
Sequences flagged as poor quality0
Sequence length35-151
%GC36

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN335920459.322912223097813No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGG489050.013.768326145
CGCGGGG523150.012.825717145
CGGGGGG1052900.012.520931145
TCGCCGG757450.011.540837145
GCGGGGG1185000.011.50395145
CGCGCGG360400.011.340987145
CGGTGGG991500.011.294712145
CGCGAGG345900.011.065065145
GCGCCGG573650.010.584629145
TCTCGGG742650.010.402869145
GGCGCGG546200.010.035195145
CCGCCGG505400.09.957124145
CCGGCGG487100.09.943179145
GCGCGCG470050.09.901745145
CCGGGGG683200.09.8556795145
CGGCGCG422250.09.679791145
GGGCGCG575700.09.603049145
CCCGCGG398300.09.550037145
AGGGGGG1609100.09.440989145
CCGCGGG393950.09.415602145
Produced by FastQC (version 0.11.5)