FastQCFastQC Report
Wed 28 Mar 2018
Geoduck-NMP-gDNA-2to4kb-5_S18_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-NMP-gDNA-2to4kb-5_S18_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences266864
Sequences flagged as poor quality0
Sequence length35-151
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN227338.51857125726962No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGGCG350.009620093130.84424145
CCGACGT552.2195841E-4124.896774145
AATAGGG950.001948363772.308655145
GAAGAGC1104.614776E-946.9978146
GATCGGA1251.4257239E-841.3665771
TCGGAAG1251.4282705E-841.3580743
CGGAAGA1455.282891E-835.653514
AGAGCAC1507.118251E-834.4650658
GCACACG803.4512585E-428.622179145
ATCGGAA1854.477315E-727.9446452
CACAGGA1350.001577403923.9340728
GGAAGAG2353.5973735E-621.9989785
GAGCACA2353.5973735E-621.9989789
AAGAGCA2404.317848E-621.5406657
TATGCCG1001.10267814E-412.1996945-49
GGGGGGG900.00498739711.329475-79
CGTCTTC1000.001371128510.96663550-54
GAACTCC950.001460912810.88370420-24
GCCGTCT1050.002058887610.44441350-54
CCGTCTT1100.0030291369.96966750-54
Produced by FastQC (version 0.11.5)