FastQCFastQC Report
Wed 28 Mar 2018
Geoduck-NMP-gDNA-2to4kb-6_S22_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-NMP-gDNA-2to4kb-6_S22_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256911
Sequences flagged as poor quality0
Sequence length35-151
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN230498.971589383093756No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTACG350.009574707131.04681145
GATCGGA1058.991783E-636.5601651
ACTCTCC750.00278755633.9924133
TAAGGGG750.00278755633.9924135
CGGAAGA1601.4162651E-731.8678874
GAAGAGC1253.043848E-530.5931746
TCGGAAG1656.478813E-627.039423
GGAAGAG1708.1696035E-626.244145
ATCGGAA1501.0449342E-425.5268272
AGAGCAC1551.3169498E-424.6719138
CCGTCTT1200.006835590619.110994145
AAGAGCA2100.001023693518.2102227
GAGCACA2200.001398519717.3824849
TTAGTAA2900.00879138613.1867127
CCCCCCC4201.6727547E-56.14793190-94
Produced by FastQC (version 0.11.5)