FastQCFastQC Report
Wed 28 Mar 2018
Geoduck-NMP-gDNA-2to4kb-6_S22_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-NMP-gDNA-2to4kb-6_S22_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256911
Sequences flagged as poor quality0
Sequence length35-151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2954611.50048071121906No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2900.11287955751213455Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGA401.4199421E-462.2035261
CGGGTAA350.00653324753.317311
ATAGAGT1350.007640067551.20305145
AGACTGG1400.0088211349.37437145
CGTATCC750.00314036933.1752131
CGTAGAA1054.5418087E-429.6207281
ATGGACG850.005933495729.1300894
CGATGCA1105.963075E-428.274331
TCGGAAG1356.2680236E-527.5178243
GATCGGA1459.874939E-525.7393911
AGTATGC1350.002017691722.941652
ATCGGAA1902.4117398E-522.8209062
AGAGCGT1450.003063346521.3500378
CGTTGTA1500.003629489620.7345091
AAGAGCG1500.003728605320.6383677
GAAGAGC1550.00451393419.9682046
CGGAAGA1906.355275E-419.5478214
GAGCGTC1650.0064704918.7621529
TGTATGG3156.231045E-515.7977221
TGTAGCA2500.003861691814.9288451
Produced by FastQC (version 0.11.5)