FastQCFastQC Report
Wed 28 Mar 2018
Geoduck-NMP-gDNA-2to4kb-7_S26_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-NMP-gDNA-2to4kb-7_S26_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498123
Sequences flagged as poor quality0
Sequence length35-151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5787511.618616285535902No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGGTG1400.005643942455.3054145
TAGCCGT1600.00956903948.392227145
TCGGAAG1953.230275E-521.9815063
GATCGGA2054.708866E-520.9329991
CGGAAGA2259.718517E-519.0506384
GAAGAGC2259.718517E-519.0506386
AGAGCAC3100.001116248513.8270778
ATCGGAA3150.001257408513.6106892
AAGAGCA3702.8085033E-413.2398247
GGAAGAG3400.00223829712.6070395
GAGCACA3600.00343551411.9066499
GCACACG2400.002702044810.753828145
TATGCCG1700.00213884457.729814545-49
GAACTCC1900.00159666967.09027520-24
ATCTCGT2050.00208617356.913004440-44
CGTCTTC2200.00299518356.679363350-54
ACGTCTG2908.29982E-56.33457215-19
TCTCGTA2350.0086847756.030493340-44
CGTCTGA2900.00363031685.48996215-19
Produced by FastQC (version 0.11.5)