FastQCFastQC Report
Wed 28 Mar 2018
Geoduck-NMP-gDNA-2to4kb-7_S26_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-NMP-gDNA-2to4kb-7_S26_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498123
Sequences flagged as poor quality0
Sequence length35-151
%GC36

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5920011.884614844124846No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGAGC2956.9710927E-414.7147027
AGAGCGT2700.00658192213.7804358
GAGCGTC2850.009410113.0551489
TCGGAAG3500.00253084112.402393
CGGAAGA3600.003125803512.0578794
ATCGGAA4100.00824017610.5874082
TCTTTTT16800.00841672610.545442145
CGCCCAC1200.00922660810.429816135-139
GCCGTAT2650.00344195999.55059145
TCGGTGG2800.0092138999.03895145
CTCGGTG3150.0057509248.034622145
CCCCCCG2150.00588624377.905459140-144
CGCCGTA2502.3918756E-46.88108445-49
TCGCCGT2553.047645E-46.746160545-49
GCGTCGT2600.00572571345.7241810-14
Produced by FastQC (version 0.11.5)