FastQCFastQC Report
Wed 28 Mar 2018
Geoduck-NMP-gDNA-2to4kb-8_S30_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-NMP-gDNA-2to4kb-8_S30_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510999
Sequences flagged as poor quality0
Sequence length35-151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5936811.61802664975861No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTGG1550.00867621349.607788145
TGTCTCG1600.00983576748.05754145
GATCGGA2051.6680133E-930.0508481
ATACCTT3900.00977002126.287888145
GCAAAAT6051.0701475E-425.418863145
GAAGAGC2501.41699275E-824.5866976
CGGAAGA2801.7826096E-924.147654
TCGGAAG2753.8957296E-822.3515433
ATCGGAA3156.9339876E-921.469372
AATAGTT8057.383847E-419.10362145
CTACATG6750.006022550618.985695145
GGAAGAG3653.7789505E-818.5242255
AGAGCAC3606.644259E-717.0740958
GAGCACA3657.675717E-716.8402049
AAGAGCA4504.1043677E-715.0252057
AATCTCG2452.526132E-410.461506145
TCTCGTA2002.0074549E-79.68292440-44
GAACTCC2406.848313E-88.70778820-24
ATGCCGT3056.397274E-58.403504145
TATGCCG2555.9032936E-78.23548745-49
Produced by FastQC (version 0.11.5)