FastQCFastQC Report
Wed 28 Mar 2018
Geoduck-NMP-gDNA-2to4kb-8_S30_L008_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-NMP-gDNA-2to4kb-8_S30_L008_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510999
Sequences flagged as poor quality0
Sequence length35-151
%GC36

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5960311.664014998072403No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCGT3250.004533451530.76524145
AGAGCGT3401.4699253E-820.1130268
GAGCGTC3451.7384082E-819.8215319
AAGAGCG3703.878631E-818.4822397
GATCGGA3504.3722866E-717.7700021
ATCGGAA3605.887414E-717.2725752
GAAGAGC3656.8176996E-717.03226
TCGGAAG3801.0362801E-616.3634913
CGTCGTG3303.944433E-415.14955145
CGGAAGA4404.760317E-614.1321064
GCAATGA3300.00159600413.1870537
GGAAGAG5051.9746503E-512.3104035
TTGTTTT26852.9306386E-510.240757145
GGTCGCC2701.860717E-78.18368840-44
GTGGTCG3559.229474E-57.04134145
CGCCGTA2656.493217E-56.986524645-49
TGGTCGC2802.230412E-56.96301240-44
TCGCCGT2902.8595281E-56.840255345-49
TCGGTGG3754.163619E-56.6658025145
GCCGTAT2851.6803922E-46.496242545-49
Produced by FastQC (version 0.11.5)