FastQCFastQC Report
Wed 28 Mar 2018
Geoduck-NMP-gDNA-3_S9_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-NMP-gDNA-3_S9_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53313
Sequences flagged as poor quality0
Sequence length35-151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN563810.575281826196237No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGCG200.0024207588206.9828145
CGGGCGG250.0047195787165.58626145
TCTGGTT250.0047195787165.58626145
GCCCTCG300.00814083137.98854145
GTATGCA250.001562753876.404232
TGTATGC400.00996774947.8538131
TTCCACC250.00890934126.799555135-139
Produced by FastQC (version 0.11.5)