FastQCFastQC Report
Wed 28 Mar 2018
Geoduck-NMP-gDNA-5to7kb-4_S15_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-NMP-gDNA-5to7kb-4_S15_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences833817
Sequences flagged as poor quality0
Sequence length35-151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10480712.569544636293095No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGGG2703.8841325E-544.98864145
ACGTGGG2450.001306248639.663452145
TGCCACG2550.001590055338.10802145
CCGCCGG2850.00274463234.096653145
CCCGCTT2850.00274463234.096653145
GTGTACG3652.2928142E-433.279266145
CGCGCCG3250.005220875629.90014145
ATCGATG3300.005625418429.44711145
CGTTCAT4800.002016956220.244886145
GCGGGGT2400.006435163320.244886145
CGTACTA2550.00413908314.7802381
CGTTCAA4257.2944746E-411.8241911
CGTTAAA5756.701246E-510.9245241
CGTAAAT5253.511786E-410.7684591
CGTTTTC5950.00111032449.5015821
CGTATTT6850.0039913488.25321
CGTTTAT7750.00133780948.1052921
GCCTTCG1900.00582495337.915194125-129
CGTATAT8550.00351149227.34690241
CCTCTAC5153.7944858E-45.3073745135-139
Produced by FastQC (version 0.11.5)