FastQCFastQC Report
Wed 28 Mar 2018
Geoduck-NMP-gDNA-5to7kb-5_S19_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-NMP-gDNA-5to7kb-5_S19_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334676537
Sequences flagged as poor quality0
Sequence length35-151
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4998412414.935054739137568No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT7207050.2153437484624146TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA3147400.030.9403511
TCGGAAG3242750.030.1636893
CGGAAGA3248400.030.0659224
GAAGAGC3367900.028.9973536
ATCGGAA3572200.027.5170692
AGAGCAC3721500.026.243748
GGAAGAG3946450.024.9227755
AAGAGCA4130350.023.8302577
GAGCACA4187250.023.4275119
AGATCGG2267700.016.2944011
CGTATGC2903200.010.40066245-49
TATGCCG2907100.010.25622645-49
CGTCTTC2982300.010.2088750-54
CCGTATC2700100.09.89873335-39
CGGCGCG133000.09.815664145
CGCGGGG164350.09.802386145
TCTCGTA2839700.09.72917240-44
TATCTCG2914300.09.57725140-44
CTTCTGC3374000.09.47230955-59
ATCTCGT2976900.09.38637440-44
Produced by FastQC (version 0.11.5)