FastQCFastQC Report
Wed 28 Mar 2018
Geoduck-NMP-gDNA-5to7kb-5_S19_L005_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-NMP-gDNA-5to7kb-5_S19_L005_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334676537
Sequences flagged as poor quality0
Sequence length35-151
%GC37

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4955928214.808113662297156No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG7400190.2211146937976115Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC4341650.022.7722289
GATCGGA4371550.022.576561
TCGGAAG4442200.022.3497893
CGGAAGA4504350.022.0170734
AGAGCGT4525000.021.9069218
AAGAGCG4592100.021.552867
ATCGGAA4675700.021.2978862
GAAGAGC4697000.021.1422186
GGAAGAG5382900.018.5796875
GCGCGGG370450.011.680821145
TCGCCGG530500.011.278481145
GGCGCGG418750.011.226536145
CGCGCGG301200.011.085186145
CGCGGGG378200.010.805823145
TCTCGGG532150.010.691373145
AGATCGG3531600.010.3438681
GCGCCGG407150.010.234299145
CCCGCGG304150.010.011634145
CGGGGGG679850.09.979495145
CCGGCGG366150.09.702431145
Produced by FastQC (version 0.11.5)