FastQCFastQC Report
Wed 28 Mar 2018
Geoduck-NMP-gDNA-5to7kb-6_S23_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-NMP-gDNA-5to7kb-6_S23_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337457885
Sequences flagged as poor quality0
Sequence length35-151
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5032100014.911786695990227No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT7042750.20870011675679173TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA3142350.030.4245151
TCGGAAG3228600.029.696023
CGGAAGA3239650.029.5651574
GAAGAGC3357500.028.550256
ATCGGAA3579050.026.9344652
AGAGCAC3701450.026.064648
GGAAGAG3957150.024.447515
AAGAGCA4128350.023.5194387
GAGCACA4190300.023.1227329
AGATCGG2256750.016.3340441
CGTATGC2906000.010.27352845-49
CGTCTTC2985300.010.10611250-54
TATGCCG2933500.010.08725345-49
GGCGCGG183650.09.977952145
CCGTATC2708400.09.79221435-39
TCTCGTA2858850.09.56296740-44
CGGGGGG228450.09.479641145
TATCTCG2927550.09.44718940-44
CTTCTGC3372450.09.38390755-59
ATCTCGT2993350.09.25374240-44
Produced by FastQC (version 0.11.5)