FastQCFastQC Report
Wed 28 Mar 2018
Geoduck-NMP-gDNA-5to7kb-7_S27_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-NMP-gDNA-5to7kb-7_S27_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences311953
Sequences flagged as poor quality0
Sequence length35-151
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN225127.216471712084833No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT5580.1788730994733181TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1650.079.499051
TCGGAAG1850.070.843253
GAAGAGC2000.065.536
AATTGCG1050.003300972563.318295145
ATCGGAA2150.060.9581382
CGGAAGA2350.055.7702144
GAGCACA2350.055.7702149
AGAGCAC2400.054.6083348
GGAAGAG2450.053.493885
AGCCTTT1250.006582667653.187366145
AAGAGCA3300.039.7151537
TCTCGTA1700.016.62501140-44
GCACACG1750.015.727200510-14
GTTCCGT1700.014.88467935-39
CCGTATC1750.014.45940335-39
CGTATCT1850.014.39769835-39
GAACTCC1850.014.1686520-24
ATCTCGT1900.014.16667640-44
AGTTCCG1705.456968E-1213.95901130-34
TATGCCG2050.013.94156645-49
Produced by FastQC (version 0.11.5)