FastQCFastQC Report
Wed 28 Mar 2018
Geoduck-NMP-gDNA-5to7kb-7_S27_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-NMP-gDNA-5to7kb-7_S27_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences311953
Sequences flagged as poor quality0
Sequence length35-151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4128613.234685994364536No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG5930.1900927383291714Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGCG1100.002718956766.47962145
CGGAAGA2000.061.3566634
AAGAGCG2200.055.7787867
AGAGCGT2200.055.7787868
GATCGGA2250.054.6403121
GACAGCG1350.00611814254.168587145
GTAGAGG1350.00611814254.168587145
TCGGAAG2300.053.3536263
GAGCGTC2300.053.3536269
GAAGAGC2600.047.1974376
GTGACTT2258.4121915E-443.334866145
GGAAGAG3050.040.2338835
ATCGGAA3050.040.2338832
CCTTCTT2550.001556932338.23665145
CGTCTTA900.00811840727.3201561
GTACCTA1650.006821213318.592932
GCCGTAT1900.013.871551545-49
CGTGTAG1800.013.63481515-19
CCGTATC2000.013.17797445-49
TGTAGGG2050.012.57063415-19
Produced by FastQC (version 0.11.5)